Cholera Toxin A Subunit from Vibrio cholerae
SIGMA/C8180 - lyophilized powder
MDL Number: MFCD00130777
Product Type: Chemical
SDS-PAGE Cholera Toxin A Subunit from Vibrio cholerae (CT) (Cat. No. C8180) was assessed on SDS-PAGE.
| availability | not available in Japan |
| composition | Protein, ~5% Lowry |
| form | lyophilized powder |
| impurities | ≤0.5% Cholera toxin B subunit (SDS-PAGE) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Cholera Toxin A Subunit from Vibrio cholerae has been used as a specific probe for trimeric G proteins in membrane motility assays. |
| Biochem/physiol Actions: | Catalyzes ADP-ribosylation of the α-subunit of G proteins, reducing GTPase activity and activating the α-subunit; also catalyzes ADP-ribosylation of cell membrane adenylyl cyclase |
| General description: | Cholera Toxin (CT) is secreted by Vibrio cholerae. CT upregulates cell surface molecules, such as co-stimulatory molecules and chemokine receptors in antigen presenting cells(APCs), murine and human dendritic cells (DCs). It enhances the secretion of interleukin 1 (IL-1) from macrophages and stimulates their APC function. CT also functions as an immunomodulator. |
| Packaging: | 0.5 mg in glass bottle |
| Physical form: | Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA |
| Preparation Note: | When reconstituted with 1 mL of water, the solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H332 - H412 |
| Precautionary statements | P261 - P271 - P273 - P304 + P340 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 21/22-52/53 |
| Safety Statements | 36/37-61 |
| RIDADR | UN 3462 6.1 / PGI |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |