Monoclonal Anti-Cdk4 antibody produced in mouse
SIGMA/C8218 - clone DCS-31, ascites fluid
MDL Number: MFCD00282839
Product Type: Chemical
Immunoblotting
HeLa cell (human) extract and Human uterine mesodermal tumor cell-SKUT extract were separated on SDS-PAGE and probed with Monoclonal Anti-CDK 4, clone DCS-31 (Cat. No. C8218). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase (Cat. No. A1293) and a NBT/BCIP substrate.
Antibody dilution 1:2,000.
HeLa cell (human) extract and Human uterine mesodermal tumor cell-SKUT extract were separated on SDS-PAGE and probed with Monoclonal Anti-CDK 4, clone DCS-31 (Cat. No. C8218). The antibody was developed with Anti-Mouse IgG (Fab), alkaline phosphatase (Cat. No. A1293) and a NBT/BCIP substrate.
Antibody dilution 1:2,000.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-31, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 33 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1:1,000 using a cultured human tumor cell line extract | |
| UniProt accession no. | P11802  |
| Application: | Monoclonal Anti-Cdk4 antibody was used in: • immunoprecipitation of human melanoma cell line. • immunofluorescence of fibrosarcoma cells. • western blot analysis • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits. |
| Biochem/physiol Actions: | The antibody reacts specifically with Cdk4 and does not recognize other Cdk types. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein. |
| General description: | Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1. |
| Immunogen: | recombinant human Cdk4 protein |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |