Chitinase from Trichoderma viride
SIGMA/C8241 - lyophilized powder, ≥600 units/g solid
Synonym: N-acetyl-β-glucosaminidase and chitodextrinase
EC Number: 232-578-7
MDL Number: MFCD00130771
Product Type: Chemical
Chitinase specificity: Chitin is a polymer of β-(1-4)-N--acetyl-D-glucosamine units
Unit Definition One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C8241-25UN
| form | lyophilized powder |
| mol wt | 30 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| solubility | 0.05 M phosphate buffer, pH 6.0: soluble 0.90-1.10 mg/mL, faintly hazy to hazy (with particles) |
| specific activity | ≥600 units/g solid |
| storage temp. | −20°C |
| Application: | Agriculture fields: control pathogens. Human health care: Asthma. Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine, Preparation of single-cell protein Isolation of protoplasts from fungi and yeast Control of pathogenic fungi Treatment of chitinous waste, mosquito control and morphogenesis |
| Biochem/physiol Actions: | Chitinase is a 30 kDa (approx.) extracellular enzyme complex that degrades chitin. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase |
| Biochem/physiol Actions: | Chitinase serves as a biopesticide against several fungi and insects. This hydrolytic enzyme is capable of cleaving the glycosidic bonds in chitin. |
| Features and Benefits: | Chitinase is an extracellular complex of enzymes that degrade chitin. It is a lytic enzyme suitable for fungal cell walls lysis. |
| General description: | Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source. The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions: • The first reaction, chitodextrinase-chitinase • The second activity is N-acetyl-glucosaminidasec |
| Other Notes: | One unit will liberate 1.0 mg of N-acetyl- One new 1 hour unit = approx. 50 old 48 hour units. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 25 units in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥600 units/g solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.14 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |