Monoclonal Anti-Cathepsin K antibody produced in mouse
SIGMA/C8243 - ~2 mg/mL, clone CL124-1H6, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-CTSK; Anti-CTSO2; Anti-PKND; Anti-PYCD
MDL Number: MFCD02097890
Product Type: Chemical
Immunoblotting Total cell extract of HCT-116 cells was separated on SDS-PAGE and incubated with serial concentrations of Monoclonal Anti-Cathepsin K (Cat. No. C8243). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 16 μg /mL antibody 2. 8 μg /mL antibody 3. 4 μg /mL antibody 4. 2 μg /mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL124-1H6, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 10-20 μg/mL using total cell extracts of HCT-116 cells | |
| UniProt accession no. | P43235  |
| Application: | Monoclonal Anti-Cathepsin K antibody produced in mouse has been used in western blotting and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Cathepsin is involved in various functions such as intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Among all the types, cathepsins K (Ctsk) is highly expressed in osteoclasts that plays a crucial role in bone remodeling and resorption. Cathepsin K protein was found in primary breast carcinoma and prostate cancer where it performs in the invasive process of prostate cancers, thyroid carcinoma, lung adenocarcinoma and breast carcinoma. Its deficency causes pycnodysostosis, a rare bone dysplasia with an osteopetrotic-like phenotype. |
| Biochem/physiol Actions: | The antibody does not cross react with the closely related lysosomal cysteine proteases cathepsins L, B, H, S, V and W. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cathepsins are proteases that normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell. Cathepsins are classified based on their functions and presence of active site such as cathepsin B, D, K, V, F etc. |
| General description: | Monoclonal Anti-Cathepsin K (mouse IgG2b isotype) is derived from the hybridoma CK124-1H6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human cathepsin K. |
| Immunogen: | recombinant human cathepsin K. The antibody epitope resides within the peptide sequence RGYREIPEGNEKAL that corresponds to amino acids 222-235. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |