Protein Kinase A catalytic subunit human
SIGMA/C8482 - >90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Synonym: Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
MDL Number: MFCD03458215
Product Type: Chemical
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| assay | >90% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | 43.5 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | >1000 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | P17612  |
| Analysis Note: | One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay). Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4. |
| Biochem/physiol Actions: | Phosphorylates a spectrum of substrates, and regulates many cellular processes. |
| Biochem/physiol Actions: | Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP. |
| Physical form: | Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% (SDS-PAGE) |
| activity | specific activity: >1000 units/mg protein |
| Storage Temp. | −70°C |
| UNSPSC | 41106305 |