Anti-Caspase 3, Active antibody produced in rabbit
SIGMA/C8487 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Activated Caspase 3 Antibody; Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit; Anti-Apopain; Anti-CPP32; Anti-Yama
MDL Number: MFCD01321906
Product Type: Chemical
Immunofluorescence HeLa cells treated with 1 μg/mL staurosporine were fixed and permeabilized with Methanol followed by Acetone. Fixed cells were stained with 1:1,000 Anti-Caspase 3, Active antibody produced in Rabbit (Cat. No. C8487). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Caspase 3 was ran on SDS-PAGE and probed with IgG Fraction AB to Caspase 3 active (Cat. No. C8487). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a ECL substrate. Antibody diluted 1/1,000
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, bovine, human, pig, canine, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine |
| microarray: suitable | |
| western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224) | |
| UniProt accession no. | P42574  |
| Application: | Anti-Caspase 3, Active antibody has been used: • for western blotting of cytochrome c for caspase activation • as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections • in Western blot analysis of activated caspase 3 • as a primary antibody in immunodetection of rat brain sections |
| Biochem/physiol Actions: | Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1. |
| General description: | Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme. |
| Immunogen: | Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH. |
| Physical form: | Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H312 - H412 |
| Precautionary statements | P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |