Cathepsin C from bovine spleen
SIGMA/C8511 - Type X, lyophilized powder, ≥5 units/mg protein
Synonym: Dipeptidyl aminopeptidase; Dipeptidyl peptidase I
CAS Number: 9032-68-2
EC Number: 232-881-4
MDL Number: MFCD00081490
Product Type: Chemical
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| application(s) | life science and biopharma |
| assay | >25% protein (biuret) |
| biological source | bovine spleen |
| composition | Protein, ≥25% biuret |
| concentration | ≥5 unit/mg protein |
| form | lyophilized powder |
| manufacturer/tradename | Sigma-Aldrich |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥5 units/mg protein |
| storage condition | OK to freeze (Unstable. Keep frozen) |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | activity assay: suitable |
| type | Type X |
| Application: | Cathepsin C from bovine spleen has been used for the in vitro enzyme activity assays. It has also been used as a digestion enzyme for in vitro myelin oligodendrocyte glycoprotein (MOG) digestion. |
| Application: | Cathepsin C has been used in a study that demonstrated the potential of a proteomics approach to identify novel proteins expressed by extravillous trophoblast and to uncover the mechanisms leading to disease states in pregnancy. Cathepsin C has also been used in a study to evaluate biodegradable thermogels. |
| Application: | The enzyme from Sigma has been used in the activation of granzyme k (Gzmk) precursor from E. coli. Granzymes are granule-stored lymphocyte serine proteases that are implicated in T- and natural killer cell-mediated cytotoxic defense reactions. |
| Biochem/physiol Actions: | Cathepsin C (Cat C) serves as the physiological activator of groups of serine proteases within immune and inflammatory cells, playing a crucial role in the defense mechanisms of an organism. It may play a role in chronic airway diseases such as asthma. Cat C also acts as a protease link between inflammation and thrombosis. Cat C participates in neutrophil recruitment and production of chemokines and cytokines in many inflammatory diseases. Cathepsin C plays a crucial role as an essential enzyme in activating granule serine proteases in cytotoxic T lymphocytes, natural killer cells (granzymes A and B), mast cells (chymase and tryptase), and neutrophils (cathepsin G, proteinase 3, and elastase). |
| Biochem/physiol Actions: | Cathespin C is a dipeptidyl aminopeptidase that can sequentially remove dipeptides from a peptide chain with an unsubstituted N-terminus. The enzyme exhibits a preference for glycine and proline as N-terminal aminoacids. Substrates that have an N-terminal lysyl or arginyl residue, or a penultimate proryl residue are not targeted by this enzyme. The endopeptidase activity requires the presence of halide ions and sulfydryl activators. |
| Disclaimer: | Unstable. Keep frozen. |
| General description: | Research Area: Cell Signaling Dipeptidyl peptidase I (DPPI), also known as cathepsin C, is an abundant lysosomal cysteine protease from the papain superfamily with a molecular weight of approximately 200 kDa. It is widely expressed in a variety of mammalian tissues, with the highest levels found in the lungs, kidneys, liver, and spleen, and relatively lower levels in the brain. DPPI is the only member of its family that is functional as a tetramer, consisting of four identical subunits, each composed of an N-terminal fragment, a heavy chain, and a light chain. It is identified as one of the multifaceted protease-processing machines, having been shown to function beyond its role as a non-specific lysosomal protease. |
| Other Notes: | One unit will produce 1 μmole of Gly-Phe-NHOH from Gly-Phe-NH2 and hydroxylamine per min at pH 6.8 at 37 °C using |
| Packaging: | 10, 25, 100 units in glass bottle |
| Physical form: | Lyophilized from a 1 M sodium chloride solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >25% protein (biuret) |
| activity | specific activity: ≥5 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.14.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |