Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse
SIGMA/C8541 - clone CY-90, ascites fluid
Synonym: Monoclonal Anti-Cytokeratin Peptide 18
MDL Number: MFCD00162250
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 1:1,600 Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in Mouse, Clone: CY-90 (Cat. No. C8541) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CY-90, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:800 using formalin-fixed, paraffin-embedded sections of human tissue |
| western blot: suitable |
| Application: | Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in: • immunofluorescence • fluorescence microscopy • confocal fluorescence microscopy • immunolabelling • histoblots |
| Biochem/physiol Actions: | Keratins help to maintain structural integrity. They also control Fas?mediated apoptosis and regulate cell size and protein synthesis. Cytokeratin 18 (CK18) plays a major role in the development of lung cancer. It acts as a therapeutic target for non-small cell lung cancer (NSCLC). CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome. |
| Biochem/physiol Actions: | The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Epithelial cells and their derivatives characteristically contain intermediate filaments (IFs) composed of about 20 related polypeptides with molecular weights between 40,000-69,000. Each epithelial tissue has a specific and stable pattern of expression of some of these cytokeratin subunits. Epithelium derived tumors maintain the expression of the cytokeratins found in the normal tissue of origin. Therefore, carcinomas can be identified and classified by immunocytochemical staining with antibodies that react specifically with cytokeratins. |
| General description: | Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cytokeratin 18 (CK18)/keratin 18 is a structural protein and is present in single-layer epithelia. It is located on human chromosome 12q13. |
| Immunogen: | human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |