Chorionic gonadotropin human
SIGMA/C8554 - lyophilized powder, suitable for mouse embryo cell culture
Synonym: Choriogonin; HCG
CAS Number: 9002-61-3
EC Number: 232-660-2
MDL Number: MFCD00130797
Product Type: Chemical
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| biological source | human urine |
| drug control | Home Office Schedule 4.2 |
| form | lyophilized powder |
| impurities | HBV,HCV and HIV, tested negative |
| mol wt | 37.9 kDa |
| apparent mol wt 37.9 kDa by SDS-PAGE | |
| potency | ≥5,000 I.U. per mg |
| Quality Level | 200  |
| shipped in | dry ice |
| solubility | H2O: 1 mL/vial |
| saline: 1 mL/vial | |
| sterility | aseptically processed |
| storage temp. | −20°C |
| technique(s) | cell culture | embryo: suitable |
| UniProt accession no. | P01215  |
| Application: | Recommended for superovulation induction in mice. |
| Biochem/physiol Actions: | Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows: Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U. Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U. Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U. Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows: Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U. Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U. |
| General description: | Glycoprotein hormone consisting of a 92-amino acid α-chain which is identical to that of LH, FSH, and TSH; and a distinct 149-amino acid β-chain. Produced by chorionic tissue and responsible for maintaining the corpus luteum during early pregnancy. Also produced by trophoblast cell neoplasms, which are marked by elevated free hCG β-chains and hyperglycosylated hCG. |
| General description: | Note: Actual molecular weight will vary from the theoretical depending upon the degree of glycosylation. Theoretical MW is 37.9 kDa based on native form which contains 2 subunits: α = 14.9 kDa (~10.2 kDa for the polypeptide and ~4.7 kDa for the carbohydrate), and β = 23.0 kDa (~16.0 kDa for the polypeptide and ~7.0 kDa for the carbohydrate). See J. Biol. Chem. 248, 6796-6827 (1973). |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H361 |
| Precautionary statements | P281 |
| Hazard Codes | Xn |
| Risk Statements | 63-62 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |