Cathepsin B from human liver
SIGMA/C8571 - buffered aqueous solution, ≥1,500 units/mg protein (E1%/280 )
Synonym: Cathepsin B1
CAS Number: 9047-22-7
MDL Number: MFCD00130748
Product Type: Chemical
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| biological source | human liver |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥1,500 units/mg protein (E |
| storage temp. | −70°C |
| UniProt accession no. | P07858  |
| Application: | Cathepsin B from human liver has been used: • as a positive control in the screening Cathepsin B inhibitor assay • in wall extension (expansin activity) assay • in microinjection human foreskin fibroblasts to test its effect on apoptosis induction |
| Biochem/physiol Actions: | Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases. |
| Biochem/physiol Actions: | Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced. |
| Biochem/physiol Actions: | Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. |
| General description: | Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal. |
| Other Notes: | One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C. |
| Packaging: | Package size based on protein content |
| Physical form: | Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,500 units/mg protein (E |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.4.22.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352200 |