Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse
SIGMA/C8616 - clone BCT-41, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD07785413
Product Type: Chemical
Immunofluorescence
HEK 293T cells, treated with LiCl (20 μg/mL), were labeled with Monoclonal Anti phospho-β-Catenin (pThr41) (Cat. No. C8616) followed by Goat Anti-Mouse IgG(Fab)-FITC (Cat. No. F5262).
HEK 293T cells, treated with LiCl (20 μg/mL), were labeled with Monoclonal Anti phospho-β-Catenin (pThr41) (Cat. No. C8616) followed by Goat Anti-Mouse IgG(Fab)-FITC (Cat. No. F5262).
Western Blotting Whole extract of cultured Human 293T cells treated with Lici (20 mM) and calyculin A (50 nM) were separated on SDS-PAGE and probed with Monoclonal Anti-phospho-BETA-Catenin (pThr41) antibody produced in Mouse, Clone: BCT-41 (Cat. No. C8616). The antibody was developed using 1:3,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:250 antibody 2. 1:100 antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BCT-41, monoclonal |
| concentration | 5-10 mg/mL (IgG fraction of antiserum) |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~94 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr41) |
| technique(s) | immunocytochemistry: 1:50 using 293 T (human embryonic kidney cell) |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 8-10 μg/mL using extract of cultured human 293T cells treated with Lici (20 mM) and calyculin A (50 nM) | |
| UniProt accession no. | P35222  |
| Application: | Monoclonal Anti-phospho-β-Catenin (pThr41) antibody produced in mouse may be used in: • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. β-Catenin activity is regulated by phosphorylation on serine/threonine residues located at the protein amino terminus.. The phosphorylation of β-catenin occurs in a protein complex that includes the APC complex (Adenomatous Polyposis Coli), together with Axin by casein kinase I (CKI) and glycogen synthase kinase-3 (GSK-3). The phosphorylation of the β-catenin protein targets the protein to degradation by the β-TrCP (β-transducin repeat-containing protein). Mutations in the serine and threonine residues in the amino-terminal region of the protein and in particular in the phosphorylation sites in that region, cause a reduction in the degradation of the protein and can lead to cancer. Monoclonal antibodies to phospho- β-catenin (pThr41) are an important tool for studying the regulation of β-catenin by phosphorylation and thus the transcription control of many proteins |
| Biochem/physiol Actions: | Monoclonal Anti-phospho-β-Catenin (pThr41) reacts specifically with human β-catenin phosphorylated at Thr41 (~ 94 kDa). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-phospho-β-Catenin (pThr41) (mouse IgG1 isotype) is derived from the BCT-41 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene. |
| Immunogen: | synthetic phosphopeptide corresponding to amino acids 38-50 (pThr41) of human β-catenin, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| WGK Germany | 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |