Cholesterol Oxidase from Streptomyces sp.
SIGMA/C8649 - lyophilized powder, ≥20 units/mg protein
Synonym: Cholesterol: oxygen oxidoreductase
CAS Number: 9028-76-6
EC Number: 232-842-1
MDL Number: MFCD00130783
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. pH Activity; 37 °C in 0.1 M buffer solution: pH 4.0–6.0, acetate; pH 5.5–8.5, K-phosphate
Figure 3. Temperature activity; in 0.1 M K-phosphate buffer, pH 7.0
Figure 4. pH Stability; 25 °C, 20-hr treatment with 50 mM buffer solution: pH 4.0–6.0, acetate; pH 6.0–8.5, K-phosphate; pH 8.5–11.0, K2CO3-NaHCO3
Figure 5. Thermal stability; 15-min. treatment with 50 mM K-phosphate buffer, pH 7.0
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C8649-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C8649-250UN
| biological source | Streptomyces sp. |
| composition | protein, 55-65% biuret |
| form | lyophilized powder |
| mol wt | ~34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| solubility | 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold) |
| specific activity | ≥20 units/mg protein |
| storage temp. | −20°C |
| Application: | Cholesterol oxidase from Streptomyces has been used in a study to assess the relationship between the micellar structure of model bile and the activity of esterase. Cholesterol oxidase from Streptomyces has also been used in a study to investigate the effects of sphingomyelin degradation on cell cholesterol oxidizability and steady-state distribution between the cell surface and the cell interior. |
| Application: | Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures. |
| Biochem/physiol Actions: | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2. |
| Biochem/physiol Actions: | Cholesterol Oxidase from microorganisms use cholesterol for carbon and energy. It is useful in diagnostic assays involving cholesterol, especially in lipid disorders. Cholesterol oxidase has an insecticidal property and aids protection against boll weevil larvae. The Streptomyces sp cholesterol oxidase has long shelf-life with good performance. |
| General description: | Cholesterol Oxidase produced from Streptomyces sp corresponds to a molecular weight of 62 kDa. It has a pH and temperature optimum of 7 and 37 °C, respectively. Cholesterol Oxidase comprises a FAD-binding and a steroid-binding domain. |
| General description: | Isoelectric point : 5.1 ± 0.1 and 5.4 ± 0.1 Michaelis constant : 4.3 x 10‾5M(Cholesterol) Inhibitors : Ionic detergents, Hg++, Ag+ Optimum pH : 6.5 − 7.0 Optimum temperature : 45 – 50°C pH Stability : pH 5.0 – 10.0 (25°C, 20hr) Thermal stability : Below 45°C (pH 7.0, 15min) |
| Other Notes: | One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization. |
| Packaging: | 100, 250 units in glass bottle |
| Physical form: | lyophilized powder containing bovine serum albumin and amino acids as stabilizers |
| Preparation Note: | CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |