Anti-Caspr2 antibody produced in rabbit
SIGMA/C8737 - affinity isolated antibody, lyophilized powder
Synonym: Anti-
MDL Number: MFCD03791625
Product Type: Chemical
Immunohistochemistry Anti-Caspr2: Cat. No. C8737: Immunohistochemical staining of Rat brain frozen sections using Anti-Caspr2 antibody, (1:200), (green). A. Caspr2 appears in pinceau structures (arrows) and diffusely in the molecular layer (MOL). Staining of the same section for Parvalbumin (red) labels Purkinje cells and demonstrates the position of the pinceau under Purkinje cells. B. Pre-incubation of the antibody with the Caspr2 peptide antigen blocks the staining, demonstrating specificity.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| mol wt | antigen 180 kDa |
| Quality Level | 200  |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:200 using rat brain membranes | |
| UniProt accession no. | Q9UHC6  |
| Application: | Anti-CASPR2 antibody has been used for immunohistochemitry and western blotting of proteins from mouse brain samples. It is also suitable for western blot analysis at a dilution of 1:200 by using rat brain membranes. |
| Biochem/physiol Actions: | CASPR2 (Contactin-associated protein-like 2) associates shaker like K+ channels in the juxtaparanodal region flanking nodes of Ranvier. It is expressed in different and distinct regions of the central nervous system (CNS) where it may plays an important role in cell recognition. CASPR and CASPR2 may interact with the actin-based cytoskeleton through cytoskeleton-associated protein 4.1B. CASPR2 is crucial for synapse formation and outgrowth of axon and dendrite. CASPR2 forms a complex with G-protein-coupled receptor 37 (GPR37) and multiple PDZ domain protein 1 (MUPP1). Disruption of the complex is linked with autism spectrum disorder (ASD). Genetic variations in CASPR2 are also associated with intellectual disability, epilepsy, schizophrenia and language disorders. It also regulates diet-induced obesity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CASPR2 (Contactin-associated protein-like 2) belongs to CASPR family and neurexin superfamily. |
| Immunogen: | peptide corresponding to amino acids residues 1315-1331 of human Caspr2. |
| Physical form: | Lyophilized from 0.4 mg/ml in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 5% sucrose, and ≤0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |