CelLytic™ B Cell Lysis Reagent
SIGMA/C8740 - For bacterial cell lysis, 10× concentrate
Synonym: bacterial cell lysis reagent
MDL Number: MFCD02097909
Product Type: Chemical
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| form | liquid |
| Quality Level | 200  |
| Application: | A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins. |
| Application: | TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells. |
| Features and Benefits: | • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme • Scalable for 1 to 25 grams of bacterial cell paste • No interference with downstream applications such as affinity chromatography, IP, and Western blotting • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses. |
| Other Notes: | 10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification. |
| Packaging: | 10, 50, 100 mL in poly bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41116134 |