Cholesterol Dehydrogenase from Nocardia sp.
SIGMA/C8743
CAS Number: 67775-34-2
MDL Number: MFCD03788777
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C8743-500UN
| form | lyophilized solid |
| mol wt | ~37 kDa by SDS-PAGE |
| Quality Level | 200  |
| recombinant | expressed in Streptomyces lividans |
| specific activity | ≥18 unit/mg solid |
| storage temp. | −20°C |
| Application: | Enzyme used in determining cholesterol in serum |
| Application: | The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains. |
| Biochem/physiol Actions: | Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay. |
| Biochem/physiol Actions: | Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5. |
| Other Notes: | One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C. |
| Packaging: | 500 units in poly bottle |
| Physical form: | Supplied as a lyophilized powder |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥18 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.B40 |
| UNSPSC | 12352204 |