Monoclonal Anti-Cytokeratin Peptide 14 antibody produced in mouse
SIGMA/C8791 - clone CKB1, ascites fluid
Synonym: Anti-CK14; Anti-EBS1; Anti-EBS1A; Anti-EBS1B; Anti-EBS1C; Anti-EBS1D; Anti-EBS3; Anti-EBS4; Anti-K14; Anti-NFJ
MDL Number: MFCD00162249
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identification of keratins in human materno-foetal interaction zone. By: Ahenkorah, J., Hottor, B., et al. in J Cell Mol Med, 2009. PubMed ID: 18466353 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1111/j.1582-4934.2008.00363.x), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin fixed, paraffin-embedded Rat tongue tissue sections were stained with 1:400 Monoclonal Anti-Cytokeratin Peptide 14 antibody produced in Mouse, Clone: CKB1 (Cat. No. C8791) followed by 1:30 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CKB1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): suitable | |
| indirect immunofluorescence: 1:200 using methacarn-fixed, paraffin-embedded human tissue sections | |
| UniProt accession no. | P02533   |
| Application: | Monoclonal Anti-Cytokeratin Peptide 14 antibody produced in mouse has been used in indirect immunofluorescence or immunoperoxidase techniques. |
| Biochem/physiol Actions: | Keratin 14 (KRT14) is associated with maintaining cellular stability. Mutation in the KRT14 gene leads to epidermolysis bullosa simplex, a genetic disorder. It is known to promote angiogenesis, cell migration and proliferation in cancer cells. |
| Biochem/physiol Actions: | The antibody reacts with myoepithelial cells in various organs (parotid, submandibular, mammary, prostate, and sweat glands) as well as with the basal layer of certain squamous epithelium (exocervical, laryngeal epithelium). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Cytokeratin Peptide 14 (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Keratin 14 (KRT14) is expressed in keratinocytes and localized to the basal epidermal layer. The gene is mapped to human chromosome 17q21 and encodes an intermediate filament. |
| Immunogen: | human callus keratins. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |