Cholesterol Oxidase from microorganisms
SIGMA/C8868 - lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli
Synonym: Cholesterol: oxygen oxidoreductase
CAS Number: 9028-76-6
EC Number: 232-842-1
MDL Number: MFCD00130783
Product Type: Chemical
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| composition | Protein, ≥15% biuret |
| form | lyophilized powder |
| mol wt | 64 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| Application: | Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease. |
| Application: | Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures. |
| Biochem/physiol Actions: | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2. |
| General description: | Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme. |
| Other Notes: | One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization. |
| Packaging: | 100, 500 units in glass bottle |
| Physical form: | Supplied as a lyophilzed powder containing sucrose |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |