Anti-phospho-Cofilin (pSer3) antibody produced in rabbit
SIGMA/C8992 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-18 kDa phosphoprotein; Anti-CFL1; Anti-Cofilin 1 (non-muscle); Anti-p18
MDL Number: MFCD04040757
Product Type: Chemical
Immunoblotting
Whole extract of human HeLa cells was separated on SDS-PAGE and blotted with Rabbit Anti-phospho-Cofilin (pSer3) antibody (Cat. No. C8992). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody 2 μg/mL
2. Antibody 2 μg/mL + phospho-cofilin peptide (a.a. 2-9 pSer3), 5 μg/mL
3. Antibody 2 μg/mL + cofilin peptide (a.a. 2-9), 5 μg/mL
4. Antibody 2 μg/mL - blot was pretreated with lambda protein phosphatase
5. Antibody 1 μg/mL
Whole extract of human HeLa cells was separated on SDS-PAGE and blotted with Rabbit Anti-phospho-Cofilin (pSer3) antibody (Cat. No. C8992). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody 2 μg/mL
2. Antibody 2 μg/mL + phospho-cofilin peptide (a.a. 2-9 pSer3), 5 μg/mL
3. Antibody 2 μg/mL + cofilin peptide (a.a. 2-9), 5 μg/mL
4. Antibody 2 μg/mL - blot was pretreated with lambda protein phosphatase
5. Antibody 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~19 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, pig (predicted), rat (predicted) |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer3) |
| technique(s) | western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells |
| UniProt accession no. | P23528  |
| Application: | Anti-phospho-Cofilin antibody was used: • as primary antibody for immunoblottingto to study the role of the Rac1 GTPase. • for western blot analysis in a comparative study of signals induced by integrin ligation during cell attachment, mechanical force from intracellular contraction, or cell stretching by external force. • as primary antibody for western blotting in a study to detect the level of cofilin. |
| Biochem/physiol Actions: | Cofilin binds stoichiometrically to monomeric G-actin and actin protomers in filaments in an apparently pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist.It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed’ ends under specific conditions. Cofilin is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. It′s activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated form, it behaves inactive and unable to bond with actin. Phosphorylation of cofilin is regulated in vertebrates by at least four protein kinases: LIM Kinase 1, LIM Kinase 2, Testicular Kinase 1, and Testicular Kinase 2. The dephosphorylation of cofilin enables its actin severing and depolymerizing activity and drives directional cell motility, thus providing a simple phosphoregulatory mechanism for actin reorganization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cofilin is a small phosphoinositide-sensitiv |
| Immunogen: | synthetic phosphopeptide corresponding to amino acids 2-9 (pSer3) of human cofilin with a C-terminal added cysteine, conjugated to KLH. This sequence is identical in mouse, rat, and pig. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |