Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
SIGMA/C9080 - clone V9, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Vimentin
Product Type: Chemical
Immunofluorescence Whole extract of Human foreskin fibroblasts HS-68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:200 Monoclonal Anti-Vimentin-Cy3 antibody produced in Mouse, Clone: V9 (Cat. No. C9080). Picture A: cells were counterstained with DAPI (blue) to stain nuclei.
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:50 Monoclonal Anti-Vimentin-Cy3 antibody produced in Mouse, Clone: V9 (Cat. No. C9080).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | V9, monoclonal |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~58 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:200 using cultured cells |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil | |
| immunohistochemistry (frozen sections): suitable using human tonsil | |
| UniProt accession no. | P08670  |
| Application: | Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in: • immunofluorescence and confocal microscopy • cytoskeleton staining • double labeling experiments • immunohistochemical • immunocytochemical localozation |
| Biochem/physiol Actions: | Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast). |
| Biochem/physiol Actions: | The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas. |
| Biochem/physiol Actions: | Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes. |
| General description: | Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells. |
| Immunogen: | pig eye lens vimentin. |
| Legal Information: | Cy is distributed under license from Amersham Biosciences Limited. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Preparation Note: | Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |