EX-CELL® ACF CHO Medium
SIGMA/C9098 - Animal-component free, without L-glutamine, use at 21.5 g/L, dry powder, suitable for cell culture
Synonym: CHO Medium
MDL Number: MFCD01634638
Product Type: Chemical
| concentration | 21.5 g/L |
| description | for research or for further manufacturing use |
| form | dry powder |
| quality | Drug/Device Master File available |
| Quality Level | 500  |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Features and Benefits: | Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity. |
| Legal Information: | EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells. |
| Other Notes: | Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants. Does not contain antibiotics, antimycotics, |
| Preparation Note: | Powdered media are extremely hygroscopic and should be protected from atmospheric moisture. Preparing a concentrated solution of medium is not recommended as precipitates may form. 1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC. 2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC. 3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2. 4. Stir until dissolved. 5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine. 6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L). 7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH. 8. Use additional water to bring the solution to final volume. 9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane. 10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |