Carboxypeptidase A from bovine pancreas
SIGMA/C9268 - (Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution
Synonym: Carboxypolypeptidase; Peptidyl-L-amino-acid hydrolase
CAS Number: 11075-17-5
MDL Number: MFCD00130718
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| form | ready-to-use solution |
| grade | Proteomics Grade |
| impurities | ≤0.05 BTEE units/mg protein chymotrypsin |
| ≤10 BAEE units/mg protein trypsin | |
| mol wt | ~35 kDa |
| purified by | 2× crystallization |
| quality | (Type II-PMSF treated) |
| Quality Level | 300  |
| specific activity | ≥50 units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by E |
| Application: | Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase. |
| Application: | The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose. |
| Biochem/physiol Actions: | Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of hippuryl- |
| Packaging: | 500, 2500, 5000 units in glass bottle |
| Preparation Note: | Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 23-45-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.17.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |