Cholesterol Esterase from Pseudomonas fluorescens
SIGMA/C9281 - lyophilized powder, ≥10,000 units/g protein
Synonym: CE; bile salt-stimulated lipase; cholesteryl ester hydrolase; pancreatic cholesterol esterase; Sterol-ester acylhydrolase
CAS Number: 9026-00-0
EC Number: 232-808-6
MDL Number: MFCD00071052
Product Type: Chemical
Stoichiometry: Cholesterol ester + H2O→ Cholesterol + Fatty acid
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C9281-500UN
| assay | 10-30% (TCA-Biuret) |
| biological source | Pseudomonas fluorescens |
| color | tan to brown |
| composition | Protein, ~20% |
| form | lyophilized powder |
| mol wt | ~129 kDa |
| pH | 7-9 |
| Quality Level | 200  |
| solubility | 0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL |
| specific activity | ≥10,000 units/g protein |
| storage condition | under inert gas (argon) |
| storage temp. | −20°C |
| technique(s) | cell based assay: suitable |
| Analysis Note: | Protein determined by biuret. |
| Application: | Cholesterol esterase from Pseudomonas fluorescens has been used in: • in cholesterol esterase assay to quantify total cholesterol from human blood serum samples • a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations • an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase • for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs) • to hydrolyze native cholesterol ester (CE) during filipin staining for detection of CE within the retinal frozen sections |
| Application: | This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories. |
| Biochem/physiol Actions: | Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis.. |
| General description: | Research area: Cell Signaling Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK). |
| Other Notes: | Contains potassium phosphate and TRITON® X-100. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate. |
| Packaging: | 100, 500 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 10-30% (TCA-Biuret) |
| activity | specific activity: ≥10,000 units/g protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |