Collagen from chicken sternal cartilage
SIGMA/C9301 - Type II (Miller), powder, BioReagent, suitable for cell culture
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
SDS-PAGE Collagen from chicken sternal cartilage (Cat. No. C9301) was assessed on SDS-PAGE.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C9301-100MG
| binding specificity | Peptide Source: Fibrinogen |
| biological source | chicken (Sternal cartilage) |
| concentration | 60-80% (biuret) |
| form | powder |
| packaging | glass bottle of 100 mg |
| glass bottle of 5 mg | |
| poly bottle of 25 mg | |
| product line | BioReagent |
| Quality Level | 300  |
| shipped in | wet ice |
| solubility | acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.) |
| storage temp. | 2-8°C |
| surface coverage | 6‑10 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| type | Type II (Miller) |
| UniProt accession no. | P02460  |
| Application: | Collagen from chicken sternal cartilage has been used: • in enzyme–linked immunosorbent assay (ELISA). • chondrocyte-mediated tissue production in vitro. • induction, treatment, and assessment of collagenα induced arthritis (CIA). • cell proliferation assay. • as a coating for cell culture surfaces. |
| Biochem/physiol Actions: | Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis. |
| Features and Benefits: | This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content. |
| General description: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types. |
| General description: | Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure. |
| Other Notes: | Major component of articular cartilage. |
| Packaging: | 100 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Preparation Note: | Prepared by a modification of the method of Trentham, D.E., et al. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |