Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit
SIGMA/C9336 - IgG fraction of antiserum, buffered aqueous solution
Synonym: CAT Antibody - Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in rabbit; Cat Antibody
MDL Number: MFCD01092314
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Western Blotting. Antibody recognition: Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in Rabbit (Cat. No. C9336) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Chloramphenicol Acetyl Transferase (CAT) (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 5 μg/mL.
Immunofluorescence 293-T cells transfected with CAT vector were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 μg/mL Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in Rabbit (Cat. No. C9336) followed by 1:50 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Picture B: cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of 293-T cells transfected with CAT vector were separated on SDS-PAGE and probed with Anti-Chloramphenicol Acetyl Transferase (CAT) antibody produced in Rabbit (Cat. No. C9336). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 10 μg/mL antibody 2. 5 μg/mL antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 26 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene |
| western blot: 10 μg/mL using eukaryotic cells transfected with a plasmid bearing the CAT gene |
| Application: | Anti-Chloramphenicol Acetyl Transferase (CAT) has been used in • immunoblotting • indirect immunofluorescence • immunofluorescence microscopy |
| Biochem/physiol Actions: | Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). |
| Biochem/physiol Actions: | Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene Anti-Chloramphenicol Acetyl Transferase (CAT) antibody is specific for bacterial CAT and recombinant CAT expressed in transfected eukaryotic cells (a predominant band of approx. 26 kD). Staining of CAT by the antibody is inhibited by the bacterial CAT antigen in cells transfected with CAT. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Chloramphenicol Acetyl Transferases (CATs) shows conservation and differences in their amino acid sequences. CAT is encode by cat gene and exists as monomer and later assemble into a trimer. |
| Immunogen: | bacterial chloramphenicol acetyl transferase (CAT). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |