Anti-Chk1 antibody, Mouse monoclonal
SIGMA/C9358 - ~2 mg/mL, clone DCS-310, purified from hybridoma cell culture
Synonym: Anti-CHK1
MDL Number: MFCD02262709
Product Type: Chemical
Western Blotting JOURNAL CITATION: LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response. By: Kalan, S., Matveyenko, A., et al. in Front Genet, 2013. PubMed ID: 23755068 Image collected and cropped by CiteAb from the following publication, (http://journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Monoclonal Anti-CHK1 Clone: DCS-310 (Cat. No. C9358). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-CHK1 Clone: DCS-310 (Cat. No. C9358). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. COS-7 4. NRK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DCS-310, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen 54 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells | |
| UniProt accession no. | O14757  |
| Application: | Monoclonal Anti-Chk1 antibody produced in mouse has been used in: • immunoprecipitation • immunohistochemistry • immunoblotting |
| Application: | Monoclonal Anti-Chk1 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoprecipitation, microarray and western blot at a working concentration of 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells. It was used for immunohistochemistry in a study to investigate the specific role of Chk1 phosphorylations in cell survival and checkpoint activation. It was used for immunohistochemistry at a working dilution of 1:4000 in a study to explore Chk1 knockdown as a novel therapeutic approach to arrest cell-cycle progression in MM cells, thus increasing the rate of cell death. It was used for the immunoprecipitation and detection of total Chk1 by western blot to study a possible regulatory relationship between Chk1 and CK1, a member of the casein kinase 1 family. |
| Biochem/physiol Actions: | Checkpoint kinase 1 (Chk1) protein acts as a cell cycle checkpoint regulator. |
| Biochem/physiol Actions: | The protein propagates signals from damaged or unreplicated DNA. It is expressed and active only in S-G2 phases of the cell cycle. In response to DNA damage, it phosphorylates CDC25C at Ser 216, thus preventing activation of the Cdc2-cyclin B complex and mitotic entry, blocking cell cycle progression. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Chk1 can phosphorylate Wee1, a negative regulator of G2 to M transition. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Checkpoint kinase 1 (Chk1) protein is a serine/threonine-protein kinase with a molar mass of 54 kDa. Monoclonal Anti-Chk1 (mouse IgG2b isotype) is derived from the DCS-310 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human Chk1. |
| Immunogen: | recombinant human Chk1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |