Anti-CUGBP2 antibody, Mouse monoclonal
SIGMA/C9367 - clone HL1889 (1H2-1F12), purified from hybridoma cell culture
Synonym: Anti-CUG Triplet Repeat, RNA-binding protein 2; Anti-ETR3, RNA-binding protein
MDL Number: MFCD09265421
Product Type: Chemical
Immunoblotting Whole extract of Mouse heart was separated on SDS-PAGE, blotted with 30 μg/mL Monoclonal Anti-CUGBP2 Clone: clone HL1889 (1H2-1F12) (Cat. No. C9367) and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HL1889 (1H2-1F12), monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~52 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 30-40 μg/mL using total cell extract of mouse heart |
| Application: | Suitable for immunocytochemistry, immunoprecipitation and western blotting at a working concentration of 30-40 μg/mL using total cell extract of mouse heart. |
| Biochem/physiol Actions: | CUGBP2 is an RNA-binding protein that specifically binds to CUG repeat sequences and is responsible for the regulation of both nuclear and cytoplasmic RNA processing events such as alternative splicing, RNA editing, mRNA stability, and translation. It is mainly expressed in muscle and brain. It regulates a specific alternative slicing of exons 5 and 21 of the N-methyl-D-aspartate receptor 1 (NMDA R1) RNA in rat. It binds to U/G-rich motifs within muscle specific enhancer elements (MSEs) and activates inclusion of an alternative exon in cardiac troponin T (cTNT) pre-mRNAs. It interacts with A/U rich sequences in the 3′ UTR and inhibits translation of cycloxygenase-2 (COX-2) mRNAs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CUGBP2 is an RNA-binding protein that specificallybinds to CUG repeat sequences. |
| Immunogen: | divergent domain of human CUGBP2 corresponding to amino acids 209-415 (accession no. NP006552). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |