Creatinine Deiminase microbial
SIGMA/C9409 - lyophilized powder, ≥25 units/mg protein
Synonym: Creatinine Deaminase
CAS Number: 37289-15-9
EC Number: 253-449-1
MDL Number: MFCD00130878
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. pH Activity; 37 °C, in Britton-Robinson buffer. The activity was assayed by the indophenol method.
Figure 3. Temperature activity; in 50 mM K-phosphate buffer, pH 7.5. The activity was assayed by the indophenol method.
Figure 4. pH Stability; 30 °C, 20-hr treatment with Britton-Robinson buffer.
Figure 5. Thermal stability; 1-hr treatment with 50 mM K-phosphate buffer, pH 7.5
| composition | Protein, ≥15% biuret |
| form | lyophilized powder |
| mol wt | ~260 kDa |
| Quality Level | 200  |
| specific activity | ≥25 units/mg protein |
| storage temp. | −20°C |
| Application: | Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples. |
| Application: | Creatinine Deiminase microbial has been used: • to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine • in creating creatinine-sensing membrane for biophysical studies • to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples |
| Biochem/physiol Actions: | Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia. |
| General description: | Isoelectric point : 4.4 Michaelis constant : 3.5 x 10‾3M (Creatinine) Structure : 6 subunits per mol of enzyme Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodo Optimum pH : 8.5 – 9.5 Optimum temperature : 65 – 75°C pH Stability : pH 7.0 – 11.0 (30°C, 20hr) Thermal stability : Below 65°C (pH 7.5, 1hr) |
| Other Notes: | One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with |
| Packaging: | 250 units in poly bottle |
| Physical form: | Lyophilized powder containing mannitol as stabilizer |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥25 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.4.21 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |