Carboxypeptidase B from porcine pancreas

SIGMA/C9584 - lyophilized powder

Synonym: Peptidyl-L-lysine(L-arginine) hydrolase; Protaminase

CAS Number: 9025-24-5
EC Number: 232-788-9
MDL Number: MFCD00081476
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-C9584-1MG	1 mg	$92.40 1/EA	Add To Favorites
45-C9584-5MG	5 mg	$321.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

biological source	Porcine pancreas
composition	Protein, 40-70%
foreign activity	carboxypeptidase A ≤1%
	chymotrypsin ≤0.1%
	trypsin ≤5%
form	lyophilized powder
grade	Proteomics Grade
InChI	1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key	TWURVFFNODFJBJ-UHFFFAOYSA-N
mol wt	34,000 Da± 600
purified by	affinity chromatography
Quality Level	200
SMILES string	S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
specific activity	≥125 units/mg protein
storage temp.	−20°C

Analysis Note:	Protein determined by biuret.
Application:	Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
Application:	The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.
Biochem/physiol Actions:	Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2’-dipyridyl.
Other Notes:	One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.
Packaging:	1, 5 mg in glass bottle
Packaging:	Package size based on protein content
Physical form:	Contains HEPES buffer salts, zinc chloride and carbohydrate
Preparation Note:	Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Symbol	GHS07,GHS08
Signal word	Danger
Hazard statements	H315 - H319 - H334 - H335
Precautionary statements	P302 + P352 - P305 + P351 + P338
Hazard Codes	Xn
Risk Statements	36/37/38-42
Safety Statements	22-24-26-36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥125 units/mg protein
Storage Temp.	−20°C
Enzyme Commission (EC) Number	3.4.17.2 ( BRENDA \| IUBMB )
UNSPSC	12352204

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