Carboxypeptidase B from porcine pancreas
SIGMA/C9584 - lyophilized powder
Synonym: Peptidyl-L-lysine(L-arginine) hydrolase; Protaminase
CAS Number: 9025-24-5
EC Number: 232-788-9
MDL Number: MFCD00081476
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C9584-1MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C9584-5MG
| biological source | Porcine pancreas |
| composition | Protein, 40-70% |
| foreign activity | carboxypeptidase A ≤1% |
| chymotrypsin ≤0.1% | |
| trypsin ≤5% | |
| form | lyophilized powder |
| grade | Proteomics Grade |
| InChI | 1S/C31H38N4O7S/c1-20(32-2 |
| InChI key | TWURVFFNODFJBJ-UHFFFAOYSA |
| mol wt | 34,000 Da± 600 |
| purified by | affinity chromatography |
| Quality Level | 200  |
| SMILES string | S(Cc3ccccc3)C(=O)C(NC(=O) |
| specific activity | ≥125 units/mg protein |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis. |
| Application: | The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis. |
| Biochem/physiol Actions: | Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulf |
| Other Notes: | One unit will hydrolyze 1.0 μmole of hippuryl- |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Contains HEPES buffer salts, zinc chloride and carbohydrate |
| Preparation Note: | Treated with protease inhibitor, AEBSF, to eliminate serine protease activity. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥125 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.17.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |