Carboxypeptidase G from Pseudomonas sp.
SIGMA/C9658 - lyophilized powder, ≥3 units/mg protein
Synonym: γ-Glutamyl hydrolase
CAS Number: 9074-87-7
EC Number: 232-978-1
MDL Number: MFCD00130719
Product Type: Chemical
Unit Definition One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C9658-20UN
| composition | Protein, ~70% biuret |
| form | lyophilized powder |
| mol wt | homodimer ~90 kDa |
| Quality Level | 300  |
| solubility | H2O: soluble 0.5 mg/mL |
| specific activity | ≥3 units/mg protein |
| storage temp. | −20°C |
| Application: | Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells. |
| Biochem/physiol Actions: | Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of |
| Packaging: | 20 units in glass bottle |
| Packaging: | 5 units in poly bottle |
| Physical form: | Contains sodium acetate salt. |
| Preparation Note: | Chromatographically purified |
| Preparation Note: | Solutions should be prepared fresh prior to use. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.17.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |