Anti-Cullin-4B antibody produced in rabbit
SIGMA/C9995 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Cul4B; Anti-MRXHF2; Anti-MRXSC; Anti-SFM2
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Cullin-4B antibody produced in Rabbit (Cat. No. C9995) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Cullin-4B (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human cullin-4B (Lanes 1-3) and of HEK-293T non transfected (Lane 4), were separated on SDS-PAGE, blotted with Anti-Cullin-4B (Cat. No. C9995) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 0.75 μg/mL 3. Antibody: 1.5 μg/mL + cullin-4B immunizing peptide (human, 40-59) 4. Antibody: 1.5 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~100 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B. |
| UniProt accession no. | Q13620  |
| Application: | Anti-Cullin-4B antibody produced in rabbit is suitable for western blotting at a working concentration of 0.7-1.5 μg/mL using HEK-293T cells expressing human Cullin-4B. The antibody was used for immunoblotting in a study to demonstrate that CUL4B E3 ubiquitin ligase plays a key role in targeting Jab1 (Jun activation domain-binding protein) for degradation and regulates bone morphogenetic protein signaling. |
| Biochem/physiol Actions: | Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases that regulate cell cycle, genome stability and DNA replication. Cul4B functions as an E3 ligase involved in selecting specific targets for ubiquitylation. It associates with damage-specific DNA binding proteins DDB1 and DDB2 (UV-damaged DNA) and functions as a substrate-recruiting factor for Cul4B E3 ligases. Cul4B ubiquitylates several target proteins including β-catenin, cyclin E, and histone H2A Mutations in this gene has been associated with x-linked mental retardation. |
| Biochem/physiol Actions: | Cullin-4B facilitates the transfer of damaged-DNA binding protein 1 (DDB1) into the nucleus independently of DDB2. In contrast to cullin-4A (Cul4A), cullin-4B (Cul4B) does not ubiquitinate DDB2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cullin-4B forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators |
| General description: | Mammalian cells express two distinct but highly homologous cullin-4 proteins, cullin-4A and cullin-4B. Cul4A and Cul4B form individual E3 ligases. In contrast to Cul4A, Cul4B has been shown to be localized to the nucleus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |