cAMP Enzyme Immunoassay Kit, Direct
SIGMA/CA200
Synonym: cAMP detection kit
Product Type: Chemical
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| Application: | cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium virulent and attenuated macrophages. It has also been used to quantify cAMP synthesis in olfactory epithelium. |
| Application: | Please refer to the attached Protocol  for details. |
| Biochem/physiol Actions: | Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states. |
| General description: | Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase. |
| General description: | Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it. |
| Other Notes: | Allow reagents to warm at room temperature for 30 minutes prior to opening. |
| Symbol |    GHS02,GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 - H311 - H314 - H330 - H335 |
| Precautionary statements | P210 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 |
| Hazard Codes | C |
| Risk Statements | 10-20/21/22-35 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 120.2 °F |
| Flash Point(C) | 49 °C |
| Supplemental Hazard Statements | EUH071 |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | 0.1 M Hydrochloric Acid 1 x 30 mL; Acetic Anhydride 1 x 1 mL; cAMP-Alkaline Phosphatase Conjugate 1 x 5 mL; cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5 mL; 5 Cycle Logit-Log Paper 1 x 1 sheets; Cyclic AMP Standard 1 x 0.5 mL; Direct cAMP Assay Layout Sheet 1 x 1 sheets; Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea; Neutralizing Reagent 1 x 6 mL; p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL; Plate Sealer 1 ea; Stop Solution 1 x 5 mL; Triethylamine 1 x 2 mL; Wash Buffer Concentrate 1 x 30 mL |