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cAMP Enzyme Immunoassay Kit

SIGMA/CA201 - sufficient for 96 assays

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-CA201-1KT 1 kit
 $0.00
1/EA
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Quality Level 200 
shipped in wet ice
storage temp. −20°C
technique(s) ELISA: suitable
usage sufficient for 96 assays
Application: cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.
General description: Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.
Other Notes: Please refer to the attached Protocol for details.
Storage Temp. −20°C
UNSPSC 12161503
Components Acetic Anhydride 1 x 1 mL; Assay Buffer 2 1 x 30 mL; cAMP-Alkaline Phosphatase Conjugate 1 x 5 mL; cAMP Assay Layout Sheet 1 x 1 sheets; cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5 mL; 5 Cycle Logit-Log Paper 1 x 1 sheets; Cyclic AMP Standard 1 x 0.5 mL; Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea; p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL; Plate Sealer 1 ea; Stop Solution 1 x 5 mL; Triethylamine 1 x 2 mL; Wash Buffer Concentrate 1 x 30 mL

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