LCL721 human cell line
SIGMA/CB_09071301 - IHW Number: 9249, 09071301
Product Type: Chemical
| biological source | human blood |
| ethnicity | Not specified |
| gender | female |
| growth mode | suspension |
| karyotype | Not specified |
| morphology | Lymphoblastoid |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Biochem/physiol Actions: | An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line with Human Leukocyte Antigen (HLA)-typing data available. |
| General description: | Consanguineous: No |
| General description: | Homozygous: No |
| Other Notes: | Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply   for more information. |
| Preparation Note: | RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) . To help establish a culture the concentration of FBS can be increased to 20%. |
| Preparation Note: | When resuscitating from frozen, cells should be seeded at 0.5 x 106 cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 105 cells/ml. Maintain cultures between 3-9 x 105 cells/ml for maximum growth; 5% CO2; 37°C. Cells form floating aggregates of varying size. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |