HeLa9903
SIGMA/CB_11033105 - 11033105, from human cervix
Synonym: HeLa
Product Type: Chemical
| biological source | human cervix |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Epithelial |
| products | Not specified |
| receptors | Not specified |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| technique(s) | cell culture | mammalian: suitable |
| Biochem/physiol Actions: | HeLa, genetic modification, the hER alpha expression construct, Oestrogen-Responsive Element |
| Biochem/physiol Actions: | HeLa9903 is a HeLa cell line stably transfected with two constructs: the hER alpha expression construct; a firefly luciferase reporter construct bearing Oestrogen-Responsive Element (ERE) driven by a mouse metallothionein (MT) promoter TATA element. HeLa9903 can be used to test for evaluation of chemicals based on OECD Test Guideline: The stably transformed human oestrogen receptor-alpha transcriptional; activation assay for detection of oestrogenic activity of chemicals. |
| Biochem/physiol Actions: | STR-PCR Data: Amelogenin: X CSF1PO: 9,10 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Legal Information: | US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Preparation Note: | DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 16μg/ml Blasticidin S HCL + 800μg/ml G418. |
| Preparation Note: | Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| UNSPSC | 41106514 |