BTB human cell line
SIGMA/CB_88052067 - IHW Number: 9067, 88052067
Product Type: Chemical
| biological source | human blood |
| ethnicity | Scandinavian |
| gender | male |
| growth mode | Suspension |
| karyotype | Not specified |
| morphology | Lymphoblastoid |
| shipped in | dry ice |
| technique(s) | cell culture | mammalian: suitable |
| Biochem/physiol Actions: | A: 201 Cw: 102 B: 27052 HLA DRA: 102 HLA DRB1: 801 HLA DQA1: 401 HLA DQB1: 402 HLA DPA1: 1 HLA DPB1: 401 |
| Biochem/physiol Actions: | This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line. |
| General description: | Consanguineous: No |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
| Other Notes: | HLA A: 2 HLA A ief: 2.2 HLA C: 1 HLA B: 27 HLA B ief: 27.5 Bw4/bw6: 4 HLA Dw: 8.1 HLA DR: 8 HLA DQ: 4.1 HLA DP: 4 |
| Preparation Note: | RPMI 1640  (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS ) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%. |
| Preparation Note: | Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium. |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B |
| WGK Germany | 3 |
| UNSPSC | 41106514 |