FLAG® M Purification Kit
SIGMA/CELLMM2 - For Mammalian expression systems.
Synonym: Anti-ddddk; Anti-dykddddk
Product Type: Chemical
FLAG® M Purification Kit workflow (Please see the attached manual for accurate instructions).
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | protein extraction: suitable |
| Application: | The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins. Learn more product details in our FLAG® application portal. |
| Features and Benefits: | • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins. • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations. • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide). |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells. |
| Packaging: | Sufficient for 3-5 uses of 1-ml affinity purification column. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |
| Components | CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.; Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powder; ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution; Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in. |