cGMP Enzyme Immunoassay Kit
SIGMA/CG201 - sufficient for 96 assays
Product Type: Chemical
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| usage | sufficient for 96 assays |
| Application: | cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues and mouse aortic artery homogenates. It has also been used to assess the guanylate cyclase activity. |
| Application: | Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well. |
| General description: | cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The immunoassay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well. |
| Symbol |   GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H302 - H311 - H314 - H330 - H335 |
| Precautionary statements | P280 - P301 + P312 + P330 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 |
| Hazard Codes | C |
| Risk Statements | 20/21/22-35 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 3316 9 |
| Supplemental Hazard Statements | EUH071 |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | Acetic Anhydride 1 x 1 mL; Assay Buffer 2 1 x 30 mL; cGMP-Alkaline Phosphatase Conjugate 1 x 5 mL; cGMP Assay Layout Sheet 1 x 1 sheets; cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5 mL; 5 Cycle Logit-Log Paper 1 x 1 sheets; Cyclic GMP Standard 1 x 0.5 mL; Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea; p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL; Plate Sealer 1 ea; Stop Solution 1 x 5 mL; Triethylamine 1 x 2 mL; Wash Buffer Concentrate 1 x 30 mL |