Glutathione Peroxidase Cellular Activity Assay Kit
SIGMA/CGP1 - Sufficient for 100 colorimetric tests
Synonym: Cellular glutathione peroxidase; c-GPx
Product Type: Chemical
Glutathione Peroxidase Assay of Blood Cell Lysates. The activity of cellular glutathione peroxidase in a rabbit reticulocyte lysate and a human erythrocyte lysate was measured using the Glutathione Peroxidase Cellular Activity Assay Kit (CGP-1). Varying amounts of both lysates were assayed for activity after a ten-fold dilution with glutathione peroxidase assay buffer.
| detection method | colorimetric |
| Quality Level | 200  |
| shelf life | Kit is stable for 24 months when unopened. |
| shipped in | wet ice |
| storage temp. | −20°C |
| usage | sufficient for 100 tests |
| Application: | Suitable for the measureent of glutathione peroxidase in tissue extracts |
| Biochem/physiol Actions: | The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions. |
| General description: | The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions. |
| Other Notes: | This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme. |
| Symbol |      GHS02,GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H226 - H242 - H302 + H332 - H311 - H314 - H341 - H411 |
| Precautionary statements | P210 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 - P370 + P378 |
| Hazard Codes | O,T,N |
| Risk Statements | 7-10-21/22-23-34-43-51/53-68 |
| Safety Statements | 3/7-17-26-36/37/39-45-61 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | tert-Butyl hydroperoxide 1 mL |