Imprint® Ultra Chromatin Optimization Kit
SIGMA/CHROP - Kit designed to optimize sonication parameters for ChIP experiments
Product Type: Chemical
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| Quality Level | 200  |
| General description: | The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical. |
| Legal Information: | Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification. |
| Symbol |      GHS02,GHS03,GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H226 - H271 - H290 - H302 - H314 - H336 - H410 |
| Precautionary statements | P210 - P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310 |
| Hazard Codes | C |
| Risk Statements | 10-22-34-67 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 100.4 °F |
| Flash Point(C) | 38 °C |
| UNSPSC | 41116158 |
| Components | IGEPAL® CA-630, Molecular Biology; Sodium chloride solution, 5 M in H2O, BioReagent, Molecular Biology, suitable for cell culture; Water, Nuclease-Free Water, for Molecular Biology; 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC); Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution; Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) |