U2OS GFP-LMNB1
SIGMA/CLL1033
Product Type: Chemical
Fluorescence Microscopy U2OS GFP-LMNB1: Cat. No. CLL1033: U2OS osteosarcoma knock-in cell line expressing the LMNB1 gene (lamin B1) endogenously tagged with GFP at the N-terminus. Cells were imaged live in Hanks Balanced Salt solution, Cat. No. H8264, supplemented with 2% Fetal Bovine Serum, Cat. No. F2442, using GFP filterset (ex 450–490/em 500–550) and 40×/1.3 oil objective.
Differential Interference Contrast (DIC) Microscopy U2OS GFP-LMNB1: Cat. No. CLL1033: Differential interference contrast (DIC) microscopy of the same cells seen in the fluorescence microscopy image.
| biological source | human female bone (Source Disease: Osteosarcoma) |
| OMIM accession no. | 169500   |
| Quality Level | 200 |
| storage temp. | −196°C |
| Analysis Note: | Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation. |
| Application: | This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of LaminB1. Fluorescence imaging shows the characteristic LaminB1 nuclear expression. This stable cell line was expanded from a single clone. The target’s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter. |
| Application: | To learn more, please visit the Cellular Reporter Cell Line webpage |
| Biochem/physiol Actions: | Lamin B1 (LMNB1) is essential for proper chromosome condensation in interphase nuclei. It also participates in the distribution of heterochromatin and modulation of gene expression and splicing. Mutations in LMNB1 (lamin B1) gene results in the adult onset demyelinating disease autosomal dominant leukodystrophy (ADLD). |
| Biochem/physiol Actions: | Nuclear lamin B1 encoded by LMNB1 gene is an intermediate filament protein of the nuclear envelope. The protein has transcriptional co-regulatory activity. It plays a key role in DNA replication, cellular aging and stress responses. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC) patients. Thus, LMNB1 can be used as a potential biomarker for HCC. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Features and Benefits: | GFP-tagged on chromosome 5q23.2 |
| Features and Benefits: | The U2OS cells are adherent, with a doubling time of approx. 29 hours. |
| General description: | LMNB1 (lamin B1) is a B type lamin protein. LMNB1 is abundantly expressed in various cell types. This gene is located on human chromosome 5q23. The nuclear lamina, an intermediate filament meshwork, is made of LmnB1, LmnB2, A type (LmnA/C) and associated inner nuclear membrane proteins. |
| General description: | This product was derived from ATCC Cat. No. HTB-96. |
| General description: | U2OS GFP-LMNB1 are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification. |
| Legal Information: | CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | U2OS GFP-LMNB1 cells |
| Preparation Note: | Media Renewal changes two to three times per week. Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere. Subculture Ratio: approx. 1:3-1:6 The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%. The cell freezing medium is DMSO 1X (Cat. No. C6164). |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |