MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-)
SIGMA/CLLS1199
Product Type: Chemical
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| biological source | human female mammary glands (Source Disease: Fibrocystic Disease) |
| OMIM accession no. | 171834   |
| 601728 |
|
| Quality Level | 200 |
| storage temp. | −196°C |
| Analysis Note: | Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation. |
| Application: | Media Renewal changes two to three times per week. Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere. Subculture Ratio: approx. 1:3-1:8 The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL. Cell freezing medium - DMSO 1X, Cat. No. C6164. |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Features and Benefits: | These MCF10A cells are adherent with a doubling time of approx. 20 hours Zinc finger nuclease (ZFN) modifications on chromosomes 10q23.3 and 3q26.3 |
| General description: | MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-) are pre-neoplastic mammary epithelial cells from a human adult female with ZFN modifications. This cell line was derived from ATCC Catalog No. CRL-10317. |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |
| Components | MCF10A CONTROL CELLS 1 vial |