Canine Osteoblasts: CnOb (Cryovial)
SIGMA/CN406-05
Product Type: Product-on-demand
Cell Applications : Left: Canine Osteoblasts, CnOb Right: von Kossa stained CnOb.
| biological source | canine bone (normal) |
| growth mode | Adherent |
| karyotype | 2n = 78 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | osteoblast |
| packaging | pkg of 500,000 cells |
| relevant disease(s) | arthritis; osteoporosis |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments |
| Biochem/physiol Actions: | Bone |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Canine osteoblasts provide an excellent model system for studies relevant to the skeletal system, including mechanisms of cancerogenesis. Canine osteoblasts have been used to define a biomarker profile of canine osteosarcoma (Milovancev, 2013), elucidate the roles of oncogenic STAT3 (Fossey, 2009, 2011) and canonical Wnt signaling (Piskun, 2013) pathways, and to develop personalized osteosarcoma treatments by testing Src inhibitor dasatinib (Davis, 2013), procaspase-3 activating compound PAC-1 (Book, 2012), HSP90 inhibitor STA-1474 (McCleese, 2009) and STAT3 inhibitor LLL12 (Couto, 2012, 2013). Characterization: Positive for bone mineralization. |
| Other Notes: | Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO |
| Preparation Note: | • 1st passage, >500,000 cells in Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 10 doublings |
| Preparation Note: | Please refer to the CnOb Culture Protocol  . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |