PCR Core kit with Taq DNA polymerase
SIGMA/CORET - PCR components in separate tubes to allow for optimization
Synonym: PCR kit; Taq DNA Polymerase kit
Product Type: Chemical
Representative packaging
| color | colorless |
| feature | dNTPs included |
| hotstart: no | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for 250 reactions |
| Application: | CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota. |
| Application: | PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters. |
| General description: | Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 °C without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity. |
| Legal Information: | No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | The following reagents are required but not provided in the kit: • Mineral Oil, M 8662 (optional) • Thermocycler • Primers • DNA to be amplified • Chloroform, C 7559 (optional) |
| Preparation Note: | Kit contains enough reagents for 250 units of Taq DNA polymerase. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |
| Components | 10X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial; 10X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial; Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial; Water, PCR Reagent 3 vial(s); Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL |