CRISPR-Lenti Non-Targeting Control Plasmid
SIGMA/CRISPR12
Product Type: Chemical
Vector Map Features
| application(s) | CRISPR |
| concentration | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| form | liquid |
| packaging | vial of 50 μL |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Functional Genomics/Screening/Target Validation • Non-targeting control, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines. To see more application data, protocols, vector maps visit sigma.com/crisprs . |
| Features and Benefits: | Serves as a non-targeting or negative experimental control (DNA format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transfection in your system with the Sigma lenti CRISPR/Cas9 plasmid. |
| General description: | The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001). Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associate |
| Legal Information: | CRISPR Use License Agreement Lentiviral and WPRE License Agreements |
| Physical form: | The Sigma CRISPR Lenti Non-Targeting Control Vector is provided as 1 μg of plasmid DNA in Tris-EDTA (TE). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |