CRISPR-Lenti Human HPRT1 Positive Control High Titer Transduction Particles
SIGMA/CRISPR13H
Product Type: Chemical
Vector Map Features
| application(s) | CRISPR |
| concentration | 5x108 VP/ml (via p24 assay) |
| form | liquid |
| packaging | vial of 200 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Functional Genomics/Screening/Target Validation • Positive control, HPRT1, CRISPR high titer lentivirus format, for creating gene knockouts/genetic modifications in cell lines. To see more application data, protocols, vector maps visit sigma.com/crispr. |
| Features and Benefits: | Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection. |
| Legal Information: | CRISPR Use License Agreement Lentiviral and WPRE License Agreements |
| Other Notes: | This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL−2) organisms in laboratory handling. Follow all published RGL−2 guidelines for laboratory handling and waste decontamination. |
| Physical form: | 200 μl virus, minimum p24 titer > 5 x 108 TU/mL |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41106609 |