Lenti CRISPR Non-Target gRNA Control Transduction Particles, High Titer (LV02 vector)
SIGMA/CRISPR15H
Product Type: Chemical
Vector Map Features
| application(s) | CRISPR |
| concentration | (5x108 TU/ml (via p24 titering assay)) |
| form | liquid |
| packaging | vial of 8 x 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Functional Genomics/Screening/Target Validation |
| General description: | The Lenti CRISPR Non-Target gRNA Control Transduction Particles (LV02 vector) include a gRNA sequence that does not target known human, mouse and rat genes. It is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-target) and Ef1a-driven Puromycin resistance cassette. This control requires Cas9 be delivered by a separate vector. Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids. |
| Legal Information: | CRISPR Use License Agreement Lentiviral, WPRE, and Evrogen Licenses |
| Other Notes: | Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41106609 |