Lenti CRISPR HPRT1 gRNA Control Transduction Particles, High Titer (LV02 vector)
SIGMA/CRISPR16H
Product Type: Chemical
Vector Map Features
| application(s) | CRISPR |
| concentration | (5x108 TU/ml (via p24 titering assay)) |
| form | liquid |
| packaging | vial of 8x25 μL (aliquots) |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Functional Genomics/Screening/Target Validation |
| General description: | The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control uses a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and Ef1a-driven Puromycin resistance casette. This control requires Cas9 be delivered by a separate vector. Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids. |
| Legal Information: | CRISPR Use License Agreement Lentiviral, WPRE, and Evrogen Licenses |
| Other Notes: | Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. |
| RIDADR | UN 3245 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41106609 |