CRISPR Activator Human Oct4 Positive Control Plasmid
SIGMA/CRISPR17 - gRNA Only Expression Plasmid
Product Type: Chemical
Vector Map Features
| application(s) | CRISPR |
| concentration | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| form | liquid |
| packaging | vial of 50 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Functional Genomics/Target Validation • Epigenetic Modification • Transcriptional Activation |
| Features and Benefits: | Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay. |
| General description: | OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control. |
| Legal Information: | CRISPR Use License Agreement Lentiviral and WPRE License Agreements |
| Other Notes: | 1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1). |
| Other Notes: | Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids. |
| Other Notes: | To order U6-gRNA plasmids, please visit sigma.com/crisprs Click here for more information on dcas9-p300 Gene Activator Expression Plasmid. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |