CRISPR GUS GAPDH Reporter Control for Monocots
SIGMA/CRISPRPL01
Product Type: Chemical
Vector Map Features
| application(s) | CRISPR |
| concentration | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| packaging | vial of 50 μL |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| selection | kanamycin |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | • To verify successful integration of T-DNA in plant genome • GUS receptor wheat gGAPDH control for monocots for Agrobacterium mediated transformation |
| Biochem/physiol Actions: | CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. |
| Features and Benefits: | • Low cost, genome editing option compared to other methods. • Easy to use • Online ordering • Ready to ship in 2 days |
| General description: | All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS Reporter CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter. Arabidopsis seedlings were germinated in 6 well tissue culture plates. The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product |
| Legal Information: | CRISPR Use License Agreement |
| Other Notes: | 1 vial containing 50ul of 20ng/ul plasmid DNA Keep reagent tubes closed when not in use. Practice aseptic lab technique to avoid DNase contamination. |
| Other Notes: | For ordering any of our custom CRISPR plant products please visit: CUSTOM ORDERING FORM |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |