β-Secretase (BACE1) Activity Detection Kit (Fluorescent)
SIGMA/CS0010 - 1 kit sufficient for 250 reactions
Synonym: BACE1 Activity Detection Kit (Fluorescent)
Product Type: Chemical
Cleavage of Amyloid Precursor Protein. Amyloid precursor protein (APP) is sequentially cleaved first by β-secretase (BACE1) and then by γ-secretase to form soluble amyloid precursor protein β (sAppβ) and the amyloid β42 peptide fragment (Aβ42). The Aβ42 fragments then aggregate and form the extracellular senile plaques common to Alzheimer’s disease.
Assay Principle: The substrate is linked to a fluorescent dye on one end and to a quenching group on its other end. The fluorescence of the substrate is significantly reduced due to intramolecular resonance energy transfer to the quenching group. Upon substrate cleavage by the enzyme, there is a disturbance of the energy transfer resulting in the enhancement of the fluorescent signal.
| Quality Level | 300  |
| shipped in | wet ice |
| storage temp. | −20°C |
| usage | kit sufficient for 250 reactions |
| Application: | The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1. |
| Biochem/physiol Actions: | BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | Fluorescent Assay Buffer 50 mL; Stop Solution 15 mL; Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL; Assay Standard 140 μL; BACE1 (β−Secretase) 300 units 100 μL |